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Mouse Monoclonal Antibody to ATG2A

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to ATG2A AO2495a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - Mouse Monoclonal Antibody to ATG2A AO2495a
    Western blot analysis using ATG2A mAb against human ATG2A (AA: 325-429) recombinant protein. (Expected MW is 37.2 kDa)
    detail
  • WB - Mouse Monoclonal Antibody to ATG2A AO2495a
    Western blot analysis using ATG2A mAb against HEK293 (1) and ATG2A (AA: 325-429)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • ICC - Mouse Monoclonal Antibody to ATG2A AO2495a
    Immunofluorescence analysis of K562 cells using ATG2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession Q2TAZ0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4H8G3
Isotype Mouse IgG1
Calculated MW 212.8kDa
Description ATG2A (Autophagy Related 2A) is a Protein Coding gene. An important paralog of this gene is ATG2B.;
Immunogen Purified recombinant fragment of human ATG2A (AA: 325-429) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
Additional Information
Gene ID 23130
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to ATG2A is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG2A {ECO:0000303|PubMed:21887408, ECO:0000312|HGNC:HGNC:29028}
Function Lipid transfer protein involved in autophagosome assembly (PubMed:28561066, PubMed:31271352, PubMed:30952800). Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (PubMed:31271352, PubMed:30952800). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PubMed:31271352, PubMed:30952800). Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed:31271352). Also regulates lipid droplets morphology and distribution within the cell (PubMed:22219374, PubMed:28561066).
Cellular Location Preautophagosomal structure membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:Q96BY7}. Lipid droplet {ECO:0000250|UniProtKB:Q96BY7}. Endoplasmic reticulum membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P53855}. Note=Localizes to endoplasmic reticulum-autophagosome contact sites.
Research Areas
Citations (0)
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References

1.Mol Biol Cell. 2012 Mar;23(5):896-909. ; 2.Acta Biochim Pol. 2011;58(3):365-74.;

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$ 385.00
Cat# AO2495a
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