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GRIK4

Purified Mouse Monoclonal Antibody

     
  • E - GRIK4 AO2594a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - GRIK4 AO2594a
    Figure 2:Western blot analysis using GRIK4 mAb against human GRIK4 (AA: extra 21-166) recombinant protein. (Expected MW is 42 kDa)
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  • WB - GRIK4 AO2594a
    Figure 3:Western blot analysis using GRIK4 mAb against HEK293 (1) and GRIK4 (AA: extra 21-166)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FCM - GRIK4 AO2594a
    Figure 6:Flow cytometric analysis of SH-SY5Y cells using GRIK4 mouse mAb (green) and negative control (red).
    detail
  • ICC - GRIK4 AO2594a
    Figure 4:Immunofluorescence analysis of Hela cells using GRIK4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
    detail
  • ICC - GRIK4 AO2594a
    Figure 5:Immunofluorescence analysis of SK-N-SH cells using GRIK4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
    detail
  • IHC - GRIK4 AO2594a
    Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GRIK4 mouse mAb with DAB staining.
    detail
  • IHC - GRIK4 AO2594a
    Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GRIK4 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession Q16099
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8H5G5
Isotype Mouse IgG2b
Calculated MW 107.2kDa
Immunogen Purified recombinant fragment of human GRIK4 (AA: extra 21-166) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2900
Other Names KA1; EAA1; GRIK; GluK4
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
ICC~~ 1/200 - 1/1000
IHC~~ 1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRIK4 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GRIK4
Synonyms GRIK
Function Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
Cellular Location Cell membrane; Multi-pass membrane protein. Postsynaptic cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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References

1.Pharmacogenomics. 2014 Aug;15(11):1451-9.2.Am J Med Genet B Neuropsychiatr Genet. 2012 Jan;159B(1):21-9.

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$ 385.00
Cat# AO2594a
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