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CHRNB4

Purified Mouse Monoclonal Antibody

     
  • E - CHRNB4 AO2641a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - CHRNB4 AO2641a
    Figure 2:Western blot analysis using CHRNB4 mAb against human CHRNB4 (AA: extra 22-236) recombinant protein. (Expected MW is 51 kDa)
    detail
  • WB - CHRNB4 AO2641a
    Figure 3:Western blot analysis using CHRNB4 mAb against HEK293 (1) and CHRNB4 (AA: extra 22-236)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FCM - CHRNB4 AO2641a
    Figure 5:Flow cytometric analysis of SK-N-SH cells using CHRNB4 mouse mAb (green) and negative control (red).
    detail
  • ICC - CHRNB4 AO2641a
    Figure 4:Immunofluorescence analysis of Hela cells using CHRNB4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P30926
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H11F2
Isotype Mouse IgG1
Calculated MW 56.4kDa
Immunogen Purified recombinant fragment of human CHRNB4 (AA: extra 22-236) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1143
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
ICC~~ 1/100 - 1/500
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHRNB4 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHRNB4
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Cellular Location Postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Research Areas
Citations (0)
citation

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References

1.Lung Cancer. 2014 Oct;86(1):85-90. 2.PLoS One. 2014 May 7;9(5):e96753.

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$ 385.00
Cat# AO2641a
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