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ELANE

Purified Mouse Monoclonal Antibody

     
  • E - ELANE AO2644a
    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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  • WB - ELANE AO2644a
    Figure 2:Western blot analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)
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  • WB - ELANE AO2644a
    Figure 3:Western blot analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - ELANE AO2644a
    Figure 4:Western blot analysis using ELANE mouse mAb against HL-60 (1), THP-1 (2), MOLT4 (3), C6 (4), and K562 (5) cell lysate.
    detail
  • FCM - ELANE AO2644a
    Figure 5:Flow cytometric analysis of K562 cells using ELANE mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P08246
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 6B6G6
Isotype Mouse IgG1
Calculated MW 28.5kDa
Immunogen Purified recombinant fragment of human ELANE (AA: 140-267) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1991
Other Names GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Dilution E~~ 1/10000
WB~~ 1/500 - 1/2000
FCM~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsELANE is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELANE
Synonyms ELA2
Function Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).
Cellular Location Cytoplasmic vesicle, phagosome. Note=Localized in phagolysosomes following ingestion of E.coli by neutrophils
Tissue Location Bone marrow cells. Neutrophil (PubMed:10947984).
Research Areas
Citations (0)
citation

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References

1.Pediatr Blood Cancer. 2015 Dec;62(12):2229-31. 2.Lung. 2014 Aug;192(4):595-9.

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$ 385.00
Cat# AO2644a
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