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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ALOX12B Antibody (C-term)   

ALOX12B Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ALOX12B Antibody (C-term) AP10099b
    ALOX12B Antibody (C-term) (Cat. #AP10099b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the ALOX12B antibody detected the ALOX12B protein (arrow).
  • IHC-P - ALOX12B Antibody (C-term) AP10099b
    ALOX12B antibody (C-term) (Cat. #AP10099b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALOX12B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ALOX12B Antibody (C-term) AP10099b
    ALOX12B Antibody (C-term) (Cat. #AP10099b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75342
Other Accession Q2KMM4, O70582, NP_001130.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 80356 Da
Antigen Region 622-652 aa
Additional Information
Gene ID 242
Other Names Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase, 11311-, Epidermis-type lipoxygenase 12, ALOX12B
Target/Specificity This ALOX12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 622-652 amino acids from the C-terminal region of human ALOX12B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALOX12B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALOX12B
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}
Tissue Location Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue
Research Areas
Citations (0)

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This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.


Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Vahlquist, A., et al. J. Invest. Dermatol. 130(2):438-443(2010)
Agarwal, S., et al. Exp. Dermatol. 18(11):939-946(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)

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Cat# AP10099b
(40 western blots)
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