PRMT7 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q9NVM4 |
Other Accession | NP_061896 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 78459 Da |
Antigen Region | 660-692 aa |
Gene ID | 54496 |
---|---|
Other Names | Protein arginine N-methyltransferase 7, 211-, Histone-arginine N-methyltransferase PRMT7, [Myelin basic protein]-arginine N-methyltransferase PRMT7, PRMT7, KIAA1933 |
Target/Specificity | This PRMT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-692 amino acids from the C-terminal region of human PRMT7. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PRMT7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PRMT7 |
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Synonyms | KIAA1933 |
Function | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. |
Cellular Location | Cytoplasm, cytosol. Nucleus |
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Background
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
References
Lee,J.H., et al. J. Biol. Chem. 280 (5), 3656-3664 (2005)
Miranda,T.B., et al. J. Biol. Chem. 279 (22), 22902-22907 (2004)
Frankel A., et al. J. Biol. Chem. 277:3537-3543(2002).
Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003).
Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001).
Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999).
Gilbreth, M., et al., PNAS 95(25):14781-14786 (1998).
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