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HA Tag Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 21
- PROTOCOLS
- BACKGROUND
Application 
| WB, CHIP, IF, E |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Other Names | Glutathione S-transferase |
|---|---|
| Target/Specificity | KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen. |
| Dilution | IF~~1:10~50 CHIP~~1:100 WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | HA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Provided below are standard protocols that you may find useful for product applications.
Background
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
References
Marco. et.al., J Biol Chem. (2009) February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.
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