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BCAT1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BCAT1 Antibody (Center) AP10147c
    All lanes : Anti-BCAT1 Antibody (Center) at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - BCAT1 Antibody (Center) AP10147c
    BCAT1 Antibody (Center) (Cat. #AP10147c) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCAT1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54687
Other Accession P54690, P24288, NP_001171563.1, NP_001171562.1, NP_005495.2, NP_001171564.1, NP_001171565.1, Q9GKM4
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42966 Da
Antigen Region 81-107 aa
Additional Information
Gene ID 586
Other Names Branched-chain-amino-acid aminotransferase, cytosolic, BCAT(c), Protein ECA39, BCAT1, BCT1, ECA39
Target/Specificity This BCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-107 amino acids from the Central region of human BCAT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCAT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCAT1
Synonyms BCT1, ECA39
Function Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Cellular Location Cytoplasm.
Tissue Location During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma
Research Areas
Citations (0)

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This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.


Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Barber, M.J., et al. PLoS ONE 5 (3), E9763 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

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$ 295.00
$ 99.00
Cat# AP10147c
(40 western blots)
Availability: In Stock
Bulk Size
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