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V5 Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - V5 Tag Antibody AP1014a
    Lanes: 1:non-transfected cells; 2:V5-tagged empty plasmid; 3:protein A (V5-tagged); 4:protein B (V5-tagged); 5:protein C (V5-tagged); 6:protein D (V5-tagged); Protocol and data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.
  • WB - V5 Tag Antibody AP1014a
    Western blot analysis of anti-V5 Tag Antibody (Cat.#AP1014a) in 12tag protein (35ug/lane). 12 Tag (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional Information
Other Names Tag from influenza hemagglutinin protein
Target/Specificity KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsV5 Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)

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Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). Abgent's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.


Dunn C, et al.J Immunol Methods. 1999 Apr 22;224(1-2):141-50.

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$ 295.00
$ 99.00
Cat# AP1014a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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