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ARL8A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARL8A Antibody (Center) AP10162C
    ARL8A Antibody (Center) (Cat. #AP10162c) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).
    detail
  • WB - ARL8A Antibody (Center) AP10162C
    ARL8A Antibody (Center) (Cat. #AP10162c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the ARL8A antibody detected the ARL8A protein (arrow).
    detail
  • IHC-P - ARL8A Antibody (Center) AP10162C
    ARL8A Antibody (Center) (Cat. #AP10162c) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARL8A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - ARL8A Antibody (Center) AP10162C
    ARL8A Antibody (Center) (Cat. #AP10162c) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q96BM9
Other Accession Q5ZKQ8, NP_620150.1
Reactivity Human, Mouse
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 21416 Da
Antigen Region 116-142 aa
Additional Information
Gene ID 127829
Other Names ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B, Novel small G protein indispensable for equal chromosome segregation 2, ARL8A, ARL10B, GIE2
Target/Specificity This ARL8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-142 amino acids from the Central region of human ARL8A.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARL8A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARL8A
Synonyms ARL10B, GIE2
Function Plays a role in lysosome motility (By similarity). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation (By similarity).
Cellular Location Late endosome membrane {ECO:0000250|UniProtKB:Q9NVJ2}. Lysosome membrane {ECO:0000250|UniProtKB:Q9CQW2}. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q9NVJ2}. Cell projection, axon {ECO:0000250|UniProtKB:Q9CQW2}. Synapse {ECO:0000250|UniProtKB:Q9CQW2} Note=Localizes with microtubules at the spindle mid-zone during mitosis. {ECO:0000250|UniProtKB:Q9NVJ2}
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)
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References

Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hofmann, I., et al. J. Cell. Sci. 119 (PT 8), 1494-1503 (2006) :
Okai, T., et al. J. Cell. Sci. 117 (PT 20), 4705-4715 (2004) :
Secombe, J., et al. J. Biol. Chem. 279(17):17126-17133(2004)

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$ 182.50
$ 70.00
Cat# AP10162C
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