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ACTB AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP10168a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP10168a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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ACTB Antibody - Product info | |
| Application | WB, IHC, FC
|
| Primary Accession | P60709 |
| Other Accession | NP_001092.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 41737 Da |
ACTB Antibody - Additional info | |
| Gene ID 60 | |
| Other Names ACTB; Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed | |
| Target/Specificity This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACTB Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACTB Antibody - Protein Information | |
| Name ACTB | |
| Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells | |
| Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs | |
ACTB Antibody - Related products
AM1021a: Beta-actin Antibody (Ascites)
AM1829a: ACTB Antibody (Ascites)
ACTB Antibody - Application data
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ACTB Antibody (Cat. #AP10168a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ACTB antibody detected the ACTB protein (arrow).
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ACTB Antibody (Cat. #AP10168a) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Western blot analysis of ACTB (arrow) using rabbit polyclonal ACTB Antibody (Cat. #AP10168a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTB gene.
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ACTB Antibody (Cat. #AP10168a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ACTB Antibody - Research Areas
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BACKGROUND
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
REFERENCES
Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010) Ambrosino, C., et al. Mol. Cell Proteomics 9(6):1352-1367(2010) Yoo, Y., et al. Oncogene 29(2):263-272(2010) Boratynska, A., et al. Acta Virol. 54(1):41-48(2010) Dugina, V., et al. J. Cell. Sci. 122 (PT 16), 2980-2988 (2009) :
