|Application ||WB, IHC-P, E|
|Other Accession||Q8VCB2, A2VE44, NP_112235.2|
|Calculated MW||78171 Da|
|Antigen Region||112-140 aa|
|Other Names||Mediator of RNA polymerase II transcription subunit 25, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78, MED25, ACID1, ARC92, PTOV2|
|Target/Specificity||This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MED25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ACID1, ARC92, PTOV2|
|Function||Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.|
|Tissue Location||Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].
Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010) : Leal, A., et al. Neurogenetics 10(4):275-287(2009) Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007) Sato, S., et al. Mol. Cell 14(5):685-691(2004) Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.