|Application ||WB, IHC-P, E|
|Other Accession||Q8VCB2, A2VE44, NP_112235.2|
|Calculated MW||78171 Da|
|Antigen Region||112-140 aa|
|Other Names||Mediator of RNA polymerase II transcription subunit 25, Activator interaction domain-containing protein 1, Activator-recruited cofactor 92 kDa component, ARC92, Mediator complex subunit 25, p78, MED25, ACID1, ARC92, PTOV2|
|Target/Specificity||This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the N-terminal region of human MED25.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MED25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ACID1, ARC92, PTOV2|
|Function||Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.|
|Tissue Location||Ubiquitously expressed. Highest levels in brain, heart, kidney, peripheral leukocytes, placenta, skeletal muscle and spleen.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].
Roupelieva, M., et al. J. Gen. Virol. 91 (PT 5), 1138-1149 (2010) : Leal, A., et al. Neurogenetics 10(4):275-287(2009) Lee, H.K., et al. EMBO J. 26(15):3545-3557(2007) Sato, S., et al. Mol. Cell 14(5):685-691(2004) Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101(8):2339-2344(2004)
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