- CITATIONS: 1
|Application ||WB, IHC-P, FC, E|
|Other Accession||Q5XIH0, Q9D0U6, A5D9C6, NP_115648.2|
|Calculated MW||28771 Da|
|Antigen Region||90-117 aa|
|Other Names||Repressor of RNA polymerase III transcription MAF1 homolog, MAF1|
|Target/Specificity||This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human MAF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MAF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.|
|Cellular Location||Nucleus. Cytoplasm.|
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
Kantidakis, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(26):11823-11828(2010)
Shor, B., et al. J. Biol. Chem. 285(20):15380-15392(2010)
Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Rollins, J., et al. Int. J. Biol. Sci. 3(5):292-302(2007)
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