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SPRED3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q2MJR0 |
| Other Accession | Q6P6N5, NP_001035987.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42670 Da |
| Antigen Region | 31-59 aa |
| Gene ID | 399473 |
|---|---|
| Other Names | Sprouty-related, EVH1 domain-containing protein 3, Spred-3, SPRED3, EVE-3 |
| Target/Specificity | This SPRED3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human SPRED3. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SPRED3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SPRED3 |
|---|---|
| Synonyms | EVE-3 |
| Function | Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase (By similarity). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q6P6N5}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q6P6N5} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SPRED3 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).
References
King, J.A., et al. J. Hepatol. 44(4):758-767(2006)
Nonami, A., et al. J. Biol. Chem. 279(50):52543-52551(2004)
Mizoguchi, M., et al. Neurogenetics 5(1):81-82(2004)
Kato, R., et al. Biochem. Biophys. Res. Commun. 302(4):767-772(2003)
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