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SPRED3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPRED3 Antibody (N-term) AP10262a
    SPRED3 Antibody (N-term) (Cat. #AP10262a) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the SPRED3 antibody detected the SPRED3 protein (arrow).
  • IHC-P - SPRED3 Antibody (N-term) AP10262a
    SPRED3 antibody (N-term) (Cat. #AP10262a) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the SPRED3 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q2MJR0
Other Accession Q6P6N5, NP_001035987.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42670 Da
Antigen Region 31-59 aa
Additional Information
Gene ID 399473
Other Names Sprouty-related, EVH1 domain-containing protein 3, Spred-3, SPRED3, EVE-3
Target/Specificity This SPRED3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human SPRED3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPRED3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms EVE-3
Function Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase.
Cellular Location Membrane; Peripheral membrane protein
Tissue Location Expression is restricted to liver.
Citations (0)

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SPRED3 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).


King, J.A., et al. J. Hepatol. 44(4):758-767(2006)
Nonami, A., et al. J. Biol. Chem. 279(50):52543-52551(2004)
Mizoguchi, M., et al. Neurogenetics 5(1):81-82(2004)
Kato, R., et al. Biochem. Biophys. Res. Commun. 302(4):767-772(2003)

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Cat# AP10262a
(40 western blots)
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