|Application ||WB, IHC-P, E|
|Calculated MW||52800 Da|
|Antigen Region||397-429 aa|
|Other Names||Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, 11411-, 60S ribosomal protein L27a histidine hydroxylase, Histone lysine demethylase MINA, MYC-induced nuclear antigen, Mineral dust-induced gene protein, Nucleolar protein 52, Ribosomal oxygenase MINA, ROX, MINA (HGNC:19441)|
|Target/Specificity||This MINA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 397-429 amino acids from the C-terminal region of human MINA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MINA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.|
|Cellular Location||Nucleus. Nucleus, nucleolus|
|Tissue Location||Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines|
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Provided below are standard protocols that you may find useful for product applications.
MINA protein is directly involved in ribosome biogenesis, most likely during the assembly process of preribosomal particles. This protein is also involved in cell proliferation. MINA may have a role in esophageal squamous cell carcinoma, colon cancer and lung cancer.
Eilbracht, J., et al., Mol. Biol. Cell 15(4):1816-1832 (2004). Teye, K., et al., Am. J. Pathol. 164(1):205-216 (2004). Tsuneoka, M., et al., J. Biol. Chem. 277(38):35450-35459 (2002).
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