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>   home   >   Products   >   Primary Antibodies   >   Dnmt3a Antibody (N-term R46)   

Dnmt3a Antibody (N-term R46)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Dnmt3a Antibody (N-term R46) AP1034d
    Western blot analysis of anti-Dnmt3a Antibody (N-term R46) (Cat.#AP1034d) in Jurkat cell line lysates (35ug/lane). Dnmt3a(arrow) was detected using the purified Pab.
  • IHC-P - Dnmt3a Antibody (N-term R46) AP1034d
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Dnmt3a antibody (N-term R46) (Cat.#AP1034d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6K1
Other Accession Q1LZ53, O88508
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 101858 Da
Antigen Region 31-61 aa
Additional Information
Gene ID 1788
Other Names DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A
Target/Specificity This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human Dnmt3a.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDnmt3a Antibody (N-term R46) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.
Cellular Location Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin
Tissue Location Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Citations (0)

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CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.


Xie, S., et al., Gene 236(1):87-95 (1999).
Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).

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$ 295.00
$ 99.00
Cat# AP1034d
(40 western blots)
Availability: In Stock
Bulk Size
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