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MBD1 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP1036b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
MBD1 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q9UIS9 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 66607 Da |
MBD1 Antibody (C-term) - Additional info | |
| Gene ID 4152 | |
| Other Names MBD1; CXXC3; PCM1; Methyl-CpG-binding domain protein 1; CXXC-type zinc finger protein 3; Methyl-CpG-binding protein MBD1; Protein containing methyl-CpG-binding domain 1 | |
| Target/Specificity This MBD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MBD1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MBD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
MBD1 Antibody (C-term) - Protein Information | |
| Name MBD1 | |
| Synonyms CXXC3, PCM1 | |
| Function Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters | |
| Cellular Location Nucleus (By similarity). Nucleus matrix (By similarity). Nucleus speckle. Chromosome. Note=Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes | |
| Tissue Location Widely expressed. | |
MBD1 Antibody (C-term) - Related products
AP1036a: MBD1 Antibody (N-term)
AP1036b: MBD1 Antibody (C-term)
DC00209: Human PCM1 cDNA Clone
LY10217a: PCM1 Over-expression Lysate
BP1036a: MBD1 Antibody (N-term) Blocking Peptide
BP1036b: MBD1 Antibody (C-term) Blocking Peptide
MBD1 Antibody (C-term) - Application data
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Western blot analysis of anti-MBD1 Pab (Cat. #AP1036b) in HeLa cell lysate. MBD1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
MBD1 Antibody (C-term) - Research Areas
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BACKGROUND
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
REFERENCES
Bhattacharya SK, Ramchandani S, Cervoni N, Szyf. M. Nature, 397 (6720):579-583 1999. Hendrich B and Bird A. Mol Cell Biol, 18: 6538-6547(1998). Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A. J Biol Chem 275 (42): 32422-32429 (2000). Bader S, Walker M, Harrison D. Br J Cancer 83(12): 1646-1649 (2000).
