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NEU4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q8WWR8 |
| Other Accession | NP_001161072.1, NP_542779.2, NP_001161074.1, NP_001161071.1, NP_001161073.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 51572 Da |
| Antigen Region | 423-452 aa |
| Gene ID | 129807 |
|---|---|
| Other Names | Sialidase-4, N-acetyl-alpha-neuraminidase 4, NEU4 |
| Target/Specificity | This NEU4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-452 amino acids from the C-terminal region of human NEU4. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEU4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEU4 |
|---|---|
| Function | Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha- (2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed:15847605, PubMed:21521691, PubMed:15213228). Hydrolyzes poly- alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons (By similarity). May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation (PubMed:21521691). Has sialidase activity toward mucin, fetuin and sialyllactose (PubMed:15847605). |
| Cellular Location | [Isoform 1]: Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Cell projection, neuron projection {ECO:0000250|UniProtKB:Q8BZL1} Note=Predominantly associates with endoplasmic reticulum membranes Only a small fraction associates with mitochondrial and plasma membranes. |
| Tissue Location | [Isoform 1]: Predominant form in liver. Also expressed in brain, kidney and colon. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
References
Bigi, A., et al. Glycobiology 20(2):148-157(2010)
Xin, X., et al. Genome Res. 19(7):1262-1269(2009)
Miyagi, T., et al. Proteomics 8(16):3303-3311(2008)
Stamatos, N.M., et al. FEBS J. 272(10):2545-2556(2005)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
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