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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Nephrin (Y1210) antibody   

Nephrin (Y1210) antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Nephrin (Y1210) antibody AP10417a
    Nephrin (Y1210) antibody (Cat. #AP10417a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).
  • WB - Nephrin (Y1210) antibody AP10417a
    Nephrin (Y1210) antibody (Cat. #AP10417a) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).
  • FC - Nephrin (Y1210) antibody AP10417a
    Nephrin Antibody (Y1210) (Cat. #AP10417a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60500
Other Accession NP_004637.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 134742 Da
Antigen Region 1191-1219 aa
Additional Information
Gene ID 4868
Other Names Nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1, NPHN
Target/Specificity This Nephrin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1191-1219 amino acids from human Nephrin.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNephrin (Y1210) antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPHS1
Synonyms NPHN
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane
Tissue Location Specifically expressed in podocytes of kidney glomeruli
Research Areas
Citations (0)

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This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010)
Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010)
Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010)
Aya, K., et al. Kidney Int. 57(2):401-404(2000)

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Cat# AP10417a
(40 western blots)
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