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ATP6V0B Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q99437 |
| Other Accession | Q2TA24, NP_001034546.1, NP_004038.1 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 21406 Da |
| Antigen Region | 104-131 aa |
| Gene ID | 533 |
|---|---|
| Other Names | V-type proton ATPase 21 kDa proteolipid subunit, V-ATPase 21 kDa proteolipid subunit, Vacuolar proton pump 21 kDa proteolipid subunit, hATPL, ATP6V0B, ATP6F |
| Target/Specificity | This ATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human ATP6V0B. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ATP6V0B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATP6V0B |
|---|---|
| Synonyms | ATP6F |
| Function | Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). |
| Cellular Location | Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250|UniProtKB:Q2TA24}; Multi-pass membrane protein |
| Tissue Location | Ubiquitous. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.
References
Lu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007)
Rojas, J.D., et al. Biochem. Biophys. Res. Commun. 320(4):1123-1132(2004)
Morel, N. Biol. Cell 95(7):453-457(2003)
Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)
Izumi, H., et al. Biochim. Biophys. Acta 1628(2):97-104(2003)
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