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ATP6V0B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP6V0B Antibody (Center) AP10432c
    ATP6V0B Antibody (Center) (Cat. #AP10432c) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).
  • IHC-P - ATP6V0B Antibody (Center) AP10432c
    ATP6V0B Antibody (Center) (Cat. #AP10432c) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP6V0B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99437
Other Accession Q2TA24, NP_001034546.1, NP_004038.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21406 Da
Antigen Region 104-131 aa
Additional Information
Gene ID 533
Other Names V-type proton ATPase 21 kDa proteolipid subunit, V-ATPase 21 kDa proteolipid subunit, Vacuolar proton pump 21 kDa proteolipid subunit, hATPL, ATP6V0B, ATP6F
Target/Specificity This ATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human ATP6V0B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP6V0B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP6V0B
Synonyms ATP6F
Function Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Cellular Location Vacuole membrane; Multi-pass membrane protein
Tissue Location Ubiquitous.
Citations (0)

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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.


Lu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007)
Rojas, J.D., et al. Biochem. Biophys. Res. Commun. 320(4):1123-1132(2004)
Morel, N. Biol. Cell 95(7):453-457(2003)
Smith, A.N., et al. Mol. Cell 12(4):801-803(2003)
Izumi, H., et al. Biochim. Biophys. Acta 1628(2):97-104(2003)

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$ 295.00
$ 99.00
Cat# AP10432c
(40 western blots)
Availability: In Stock
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