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CTLA4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CTLA4 Antibody (N-term) AP10465A
    All lanes : Anti- CTLA4 Antibody (N-term) at 1:2000 dilution Lane 1: WiDr whole cell lysate Lane 2: Raji whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CTLA4 Antibody (N-term) AP10465A
    All lanes : Anti- CTLA4 Antibody (N-term) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: U266B1 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: CCRF-CEM whole cell lysate Lane 6: Human spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CTLA4 Antibody (N-term) AP10465A
    CTLA4 Antibody (N-term) (Cat. #AP10465a) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTLA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P16410
Other Accession NP_005205.2, NP_001032720.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24656 Da
Antigen Region 50-78 aa
Additional Information
Gene ID 1493
Other Names Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4, CD152
Target/Specificity This IDDM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the N-terminal region of human IDDM12.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCTLA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CTLA4
Synonyms CD152
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Cellular Location Cell membrane; Single-pass type I membrane protein Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;
Tissue Location Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation
Research Areas
Citations (0)

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Background

CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.

References

Liu, Y., et al. Hum. Immunol. 71(11):1141-1146(2010)
Andersen, M.K., et al. Diabetes Care 33(9):2062-2064(2010)
Azarpira, N., et al. Exp Clin Transplant 8(3):210-213(2010)
Liu, G., et al. Nan Fang Yi Ke Da Xue Xue Bao 30(8):1838-1840(2010)
Oaks, M.K., et al. Cell. Immunol. 201(2):144-153(2000)

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$ 295.00
$ 99.00
Cat# AP10465A
Size:
Quantity:
(40 western blots)
Availability: In Stock
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