|Application ||WB, IHC-P, E|
|Other Accession||NP_005205.2, NP_001032720.1|
|Calculated MW||24656 Da|
|Antigen Region||50-78 aa|
|Other Names||Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4, CD152, IDDM12|
|Target/Specificity||This IDDM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids of human IDDM12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CTLA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;|
|Tissue Location||Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation|
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Provided below are standard protocols that you may find useful for product applications.
CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Liu, Y., et al. Hum. Immunol. 71(11):1141-1146(2010)
Andersen, M.K., et al. Diabetes Care 33(9):2062-2064(2010)
Azarpira, N., et al. Exp Clin Transplant 8(3):210-213(2010)
Liu, G., et al. Nan Fang Yi Ke Da Xue Xue Bao 30(8):1838-1840(2010)
Oaks, M.K., et al. Cell. Immunol. 201(2):144-153(2000)
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