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ADORA2A Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ADORA2A Antibody (Center) AP10467C
    Confocal immunofluorescent analysis of ADORA2A Antibody (Center) (Cat#AP10467c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - ADORA2A Antibody (Center) AP10467C
    ADORA2A Antibody (Center) (Cat. #AP10467c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the ADORA2A antibody detected the ADORA2A protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P29274
Other Accession P30543, Q60613, NP_000666.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44707 Da
Antigen Region 273-301 aa
Additional Information
Gene ID 135
Other Names Adenosine receptor A2a, ADORA2A, ADORA2
Target/Specificity This ADORA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the Central region of human ADORA2A.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADORA2A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADORA2A
Synonyms ADORA2
Function Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P30543}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P30543} Note=Colocalizes with GAS2L2 at neuronal processes {ECO:0000250|UniProtKB:P30543}
Research Areas
Citations (0)
citation

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Background

ADORA2A is a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. This protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

References

Buira, S.P., et al. J. Neurochem. 115(1):283-295(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Rogers, P.J., et al. Neuropsychopharmacology 35(9):1973-1983(2010)
Tebano, M.T., et al. ScientificWorldJournal 10, 1768-1782 (2010) :
Kobayashi, H., et al. Behav Brain Funct 6, 50 (2010) :

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Discontinued
Cat# AP10467C
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