|Application ||WB, E|
|Calculated MW||47506 Da|
|Antigen Region||258-286 aa|
|Other Names||Transcription factor E2F2, E2F-2, E2F2|
|Target/Specificity||This E2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the Central region of human E2F2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||E2F2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.|
|Tissue Location||Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples|
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Provided below are standard protocols that you may find useful for product applications.
E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq].
Revenko, A.S., et al. Mol. Cell. Biol. 30(22):5260-5272(2010)
Hayami, S., et al. Mol. Cancer 9, 59 (2010) :
Chen, J., et al. Cancer Causes Control 20(9):1769-1777(2009)
Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
Lal, A., et al. Mol. Cell 35(5):610-625(2009)
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