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COL5A1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - COL5A1 Antibody (N-term) AP10487A
    Anti-COL5A1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - COL5A1 Antibody (N-term) AP10487A
    COL5A1 Antibody (N-term) (Cat. #AP10487a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).
  • WB - COL5A1 Antibody (N-term) AP10487A
    COL5A1 Antibody (N-term) (Cat. #AP10487a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).
  • IHC-P - COL5A1 Antibody (N-term) AP10487A
    COL5A1 antibody (N-term) (Cat. #AP10487a) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P20908
Other Accession NP_000084.3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 183560 Da
Antigen Region 54-82 aa
Additional Information
Gene ID 1289
Other Names Collagen alpha-1(V) chain, COL5A1
Target/Specificity This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL5A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL5A1
Function Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Research Areas
Citations (0)

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Background

COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq].

References

Vitart, V., et al. Hum. Mol. Genet. 19(21):4304-4311(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Posthumus, M., et al. Med Sci Sports Exerc (2010) In press :
Borck, G., et al. Am. J. Med. Genet. A 152A (8), 2090-2093 (2010) :

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$ 295.00
$ 99.00
Cat# AP10487A
Size:
Quantity:
(40 western blots)
Availability: In Stock
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