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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   NCALD Antibody (C-term)   

NCALD Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NCALD Antibody (C-term) AP10495b
    NCALD Antibody (C-term) (Cat. #AP10495b) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the NCALD antibody detected the NCALD protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P61601
Other Accession Q7SY75, Q5PQN0, Q91X97, Q4R4N4, P62758, P61602, P37235, P84076, Q06AT1, P84075, P84074, Q4PL64, NP_001035715.1, NP_001035720.1
Reactivity Human, Mouse
Predicted Bovine, Pig, Rat, Chicken, Monkey, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22245 Da
Antigen Region 147-174 aa
Additional Information
Gene ID 83988
Other Names Neurocalcin-delta, NCALD
Target/Specificity This NCALD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-174 amino acids from the C-terminal region of human NCALD.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCALD Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCALD
Function May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.
Tissue Location Retina, cerebrum, cerebellum, brain stem, spinal cord, testis, ovary and small intestine
Research Areas
Citations (0)

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Background

NCALD encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
Gill, M.B., et al. J. Biol. Chem. 284(21):14503-14512(2009)
Venkataraman, V., et al. Biochemistry 47(25):6590-6601(2008)
Kamiyama, M., et al. Hum. Genet. 122 (3-4), 397-407 (2007) :

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$ 295.00
$ 99.00
Cat# AP10495b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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