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>   home   >   Products   >   Primary Antibodies   >   Microbiology   >   WDR48 Antibody (C-term)   

WDR48 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WDR48 Antibody (C-term) AP10505b
    WDR48 Antibody (C-term) (Cat. #AP10505b) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the WDR48 antibody detected the WDR48 protein (arrow).
  • IHC-P - WDR48 Antibody (C-term) AP10505b
    WDR48 antibody (C-term) (Cat. #AP10505b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WDR48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - WDR48 Antibody (C-term) AP10505b
    WDR48 Antibody (C-term) (Cat. #AP10505b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q8TAF3
Other Accession Q4R2Z6, Q32PG3, NP_065890.1
Reactivity Human
Predicted Bovine, Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 76210 Da
Antigen Region 603-630 aa
Additional Information
Gene ID 57599
Other Names WD repeat-containing protein 48, USP1-associated factor 1, WD repeat endosomal protein, p80, WDR48, KIAA1449, UAF1
Target/Specificity This WDR48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-630 amino acids from the C-terminal region of human WDR48.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR48 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR48
Synonyms KIAA1449, UAF1
Function Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 and USP46 (PubMed:18082604, PubMed:19075014, PubMed:26388029). Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed:18082604). Also activates deubiquitinating activity of complexes containing USP12 (PubMed:19075014). Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase- interacting protein (TIP). Subsequently, TIP recruits tyrosine- protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.
Cellular Location Nucleus. Cytoplasm. Lysosome. Late endosome. Note=Mainly in cytoplasmic compartments (PubMed:12196293, PubMed:18032488). In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11 (PubMed:18032488)
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Background

Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.

References

Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010)
Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)
Cote-Martin, A., et al. J. Virol. 82(3):1271-1283(2008)
Cohn, M.A., et al. Mol. Cell 28(5):786-797(2007)
Park, J., et al. J. Virol. 77(16):9041-9051(2003)

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Cat# AP10505b
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