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CF150 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - CF150 Antibody  (Center) AP10510c
    Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - CF150 Antibody  (Center) AP10510c
    Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green, Cat#AP10510c) compared to an isotype control of rabbit IgG(blue). AP10510c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - CF150 Antibody  (Center) AP10510c
    Western blot analysis of lysate from THP-1 cell line, using CF150 Antibody (Center)(Cat. #AP10510c). AP10510c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - CF150 Antibody  (Center) AP10510c
    Western blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right), using CF150 Antibody (Center)(Cat. #AP10510c). AP10510c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - CF150 Antibody  (Center) AP10510c
    Western blot analysis of lysates from HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right), using CF150 Antibody (Center)(Cat. #AP10510c). AP10510c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • FC - CF150 Antibody  (Center) AP10510c
    CF150 Antibody (Center) (Cat. #AP10510c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q8N884
Other Accession NP_612450.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58814 Da
Antigen Region 266-295 aa
Additional Information
Gene ID 115004
Other Names Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, Mab-21 domain-containing protein 1, MB21D1, C6orf150
Target/Specificity This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
Dilution FC~~1:10~50
WB~~1:500-1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCF150 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MB21D1
Synonyms C6orf150
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. cGAMP can be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but TMEM173/STING-dependent manner (PubMed:26229115).
Cellular Location Cytoplasm, cytosol Note=Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1) (PubMed:26048138)
Tissue Location Expressed in the monocytic cell line THP1.
Research Areas
Citations ( 0 )

Background

The exact function of C6orf150 remains unknown.

References

Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)

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$ 295.00
$ 99.00
Cat# AP10510c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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