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DEN2D Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP10531b 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP10531b-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
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DEN2D Antibody (C-term) - Product info

ApplicationWB, IHC, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionQ9H6A0
Other AccessionNP_079177.2
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW53672 Da

DEN2D Antibody (C-term) - Additional info

Gene ID 79961
Other Names
DENND2D; DENN domain-containing protein 2D
Target/Specificity
This DEN2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 450-479 amino acids from the C-terminal region of human DEN2D.
Dilution
WB~~1:100~500
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
DEN2D Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

DEN2D Antibody (C-term) - Protein Information

Name DENND2D
Function
Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP- bound form

DEN2D Antibody (C-term) - Related products

AP10531b: DEN2D Antibody (C-term)

DC01524: Human DENND2D cDNA Clone

BP10531b: DEN2D Antibody (C-term) Blocking Peptide

DEN2D Antibody (C-term) - Application data

  • DEN2D Antibody (C-term) (Cat. #AP10531b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DEN2D antibody detected the DEN2D protein (arrow).

  • DEN2D antibody (C-term) (Cat. #AP10531b) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the DEN2D antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

  • DEN2D Antibody (C-term) (Cat. #AP10531b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

DEN2D Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

REFERENCES

Bloethner, S., et al. Genes Chromosomes Cancer 47(12):1076-1085(2008) Lamesch, P., et al. Genomics 89(3):307-315(2007)