SPIRE2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q8WWL2 |
Other Accession | Q8K1S6, NP_115827.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 79671 Da |
Antigen Region | 285-313 aa |
Gene ID | 84501 |
---|---|
Other Names | Protein spire homolog 2, Spir-2, SPIRE2, KIAA1832, SPIR2 |
Target/Specificity | This SPIRE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human SPIRE2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SPIRE2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SPIRE2 |
---|---|
Synonyms | KIAA1832, SPIR2 |
Function | Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (By similarity). Required for asymmetric spindle positioning and asymmetric cell division during meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PubMed:26287480). |
Cellular Location | Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8K1S6}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8K1S6}. Cell membrane {ECO:0000250|UniProtKB:Q8K1S6}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8K1S6}; Cytoplasmic side {ECO:0000250|UniProtKB:Q8K1S6}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q8K1S6}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8K1S6}; Cytoplasmic side {ECO:0000250|UniProtKB:Q8K1S6}. Note=Detected at the cleavage furrow during asymmetric oocyte division and polar body extrusion {ECO:0000250|UniProtKB:Q8K1S6} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Acts as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport (By similarity).
References
Pechlivanis, M., et al. J. Biol. Chem. 284(37):25324-25333(2009)
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