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HFM1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HFM1 Antibody (C-term) AP10614b
    HFM1 Antibody (C-term) (Cat. #AP10614b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HFM1 antibody detected the HFM1 protein (arrow).
  • IHC-P - HFM1 Antibody (C-term) AP10614b
    HFM1 antibody (C-term) (Cat. #AP10614b) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HFM1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HFM1 Antibody (C-term) AP10614b
    HFM1 Antibody (C-term) (Cat. #AP10614b) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A2PYH4
Other Accession NP_001017975.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 162610 Da
Antigen Region 1293-1321 aa
Additional Information
Gene ID 164045
Other Names Probable ATP-dependent DNA helicase HFM1, SEC63 domain-containing protein 1, HFM1, SEC3D1
Target/Specificity This HFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1293-1321 amino acids from the C-terminal region of human HFM1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHFM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HFM1
Synonyms SEC3D1
Function Required for crossover formation and complete synapsis of homologous chromosomes during meiosis.
Tissue Location Preferentially expressed in testis and ovary.
Research Areas
Citations (0)

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Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Tanaka, K., et al. DNA Seq. 17(3):242-246(2006)

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Cat# AP10614b
(40 western blots)
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