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Mouse Dnmt2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Dnmt2 Antibody (N-term) AP1061a
    Western blot analysis of anti-mDnmt2 Pab (Cat. #AP1061a) in HL-60 cell lysate. mDnmt2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - Mouse Dnmt2 Antibody (N-term) AP1061a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O55055
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46794 Da
Antigen Region 201-230 aa
Additional Information
Gene ID 13434
Other Names tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog MmuIIP, DNA MTase homolog MmuIIP, MMmuIIP, Met-2, Trdmt1, Dnmt2, Met2
Target/Specificity This Mouse Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the N-terminal region of mouse Dnmt2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Dnmt2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Trdmt1
Synonyms Dnmt2, Met2
Function Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
Cellular Location Nucleus.
Tissue Location Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells
Citations (0)

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mDnmt2 does not appear to be active as a DNA methyltransferase; however, its strong binding to DNA suggests that it may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. mDnmt2 is strongly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells. This protein belongs to the 5-cystosine methyltransferase family.


Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).
Okano, M., et al., Nucleic Acids Res. 26(11):2536-2540 (1998).

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Cat# AP1061a
(40 western blots)
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