|Application ||WB, IHC-P, E|
|Other Accession||Q99MI7, Q8C878, Q7ZVX6|
|Calculated MW||51852 Da|
|Antigen Region||415-445 aa|
|Other Names||NEDD8-activating enzyme E1 catalytic subunit, 632-, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, UBA3, UBE1C|
|Target/Specificity||This UBE1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-445 amino acids from the C-terminal region of human UBE1C.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE1C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Bohnsack, R.N., et al., J. Biol. Chem. 278(29):26823-26830 (2003).
Walden, H., et al., Nature 422(6929):330-334 (2003).
Gong, L., et al., J. Biol. Chem. 274(17):12036-12042 (1999).
Gubin, A.N., et al., Genomics 59(2):168-177 (1999).
Osaka, F., et al., Genes Dev. 12(15):2263-2268 (1998).
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