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CEP70 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CEP70 Antibody (Center) AP10646c
    CEP70 Antibody (Center) (Cat. #AP10646c) western blot analysis in WiDr,CHO,A549,U251 cell line lysates (35ug/lane).This demonstrates the CEP70 antibody detected the CEP70 protein (arrow).
    detail
  • IHC-P - CEP70 Antibody (Center) AP10646c
    CEP70 antibody (Center) (Cat. #AP10646c) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CEP70 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - CEP70 Antibody (Center) AP10646c
    CEP70 Antibody (Center) (Cat. #AP10646c) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q8NHQ1
Other Accession Q4R7V1, NP_077817.2
Reactivity Human, Hamster
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 69752 Da
Antigen Region 309-338 aa
Additional Information
Gene ID 80321
Other Names Centrosomal protein of 70 kDa, Cep70, p10-binding protein, CEP70, BITE
Target/Specificity This CEP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the Central region of human CEP70.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCEP70 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CEP70
Synonyms BITE
Function Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localized at the center of the radial microtubule array in interphase and at the spindle poles during various stages of mitosis
Research Areas
Citations (0)
citation

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References

Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Lim, J., et al. Cell 125(4):801-814(2006)
Petretti, C., et al. EMBO Rep. 7(4):418-424(2006)
Andersen, J.S., et al. Nature 426(6966):570-574(2003)
Casenghi, M., et al. Dev. Cell 5(1):113-125(2003)

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$ 182.50
$ 70.00
Cat# AP10646c
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