- CITATIONS: 1
|Application ||WB, E|
|Other Accession||O35780, O35185, Q5EA15, NP_003661.1, B3F209|
|Calculated MW||45510 Da|
|Antigen Region||22-51 aa|
|Other Names||Class E basic helix-loop-helix protein 40, bHLHe40, Class B basic helix-loop-helix protein 2, bHLHb2, Differentially expressed in chondrocytes protein 1, DEC1, Enhancer-of-split and hairy-related protein 2, SHARP-2, Stimulated by retinoic acid gene 13 protein, BHLHE40, BHLHB2, DEC1, SHARP2, STRA13|
|Target/Specificity||This BHLHE40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human BHLHE40.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BHLHE40 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BHLHB2, DEC1, SHARP2, STRA13|
|Function||Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Predominantly localized in the nucleus|
|Tissue Location||Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach|
Provided below are standard protocols that you may find useful for product applications.
BHLHE40 encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differentiation.
Wang, W., et al. Biochem. Biophys. Res. Commun. 401(3):422-428(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Soria, V., et al. Neuropsychopharmacology 35(6):1279-1289(2010)
Utge, S.J., et al. PLoS ONE 5 (2), E9259 (2010) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
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