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APG8a/b(MAP1LC3A/B) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • IF - APG8a/b(MAP1LC3A/B) Antibody AP10648a
    Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP10648a APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
  • WB - APG8a/b(MAP1LC3A/B) Antibody AP10648a
    APG8a/b (MAP1LC3A/B) (Cat. #AP10648a) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow).
  • IHC-P - APG8a/b(MAP1LC3A/B) Antibody AP10648a
    Cleaved-APG8a/b antibody (MAP1LC3A/B) (Cat. #AP10648a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZQ8
Other Accession O41515, NP_073729.1
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14688 Da
Antigen Region 100-125 aa
Additional Information
Gene ID 81631
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
Target/Specificity This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b.
Dilution IF~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPG8a/b(MAP1LC3A/B) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MAP1ALC3
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Tissue Location Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Research Areas
Citations (0)

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The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.


References for protein
1.Rouschop, K.M., et al. J. Clin. Invest. 120(1):127-141(2010)
2.Kirkin, V., et al. Mol. Cell 33(4):505-516(2009)
3.Othman, E.Q., et al. J. Clin. Lab. Anal. 23(4):249-258(2009)
4.Liu, Q., et al. Ai Zheng 27(1):25-29(2008)
5.Komatsu, M., et al. Cell 131(6):1149-1163(2007)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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$ 295.00
$ 99.00
Cat# AP10648a
(40 western blots)
Availability: In Stock
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