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>   home   >   Products   >   Primary Antibodies   >   BHLHA15 Antibody (C-term)   

BHLHA15 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BHLHA15 Antibody (C-term) AP10649B
    BHLHA15 Antibody (C-term) (Cat. #AP10649b) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the BHLHA15 antibody detected the BHLHA15 protein (arrow).
  • WB - BHLHA15 Antibody (C-term) AP10649B
    BHLHA15 Antibody (C-term) (Cat. #AP10649b) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the BHLHA15 antibody detected the BHLHA15 protein (arrow).
  • FC - BHLHA15 Antibody (C-term) AP10649B
    BHLHA15 Antibody (C-term) (Cat. #AP10649b) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q7RTS1
Other Accession NP_803238.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20818 Da
Antigen Region 160-189 aa
Additional Information
Gene ID 168620
Other Names Class A basic helix-loop-helix protein 15, bHLHa15, Class B basic helix-loop-helix protein 8, bHLHb8, Muscle, intestine and stomach expression 1, MIST-1, BHLHA15, BHLHB8, MIST1
Target/Specificity This BHLHA15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the C-terminal region of human BHLHA15.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBHLHA15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BHLHA15
Synonyms BHLHB8, MIST1
Function Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E- box occupancy by homodimers. May also negatively regulate bHLH- mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E- box consensus sequence 5'-CANNTG-3' (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in brain, liver, spleen and skeletal muscle.
Citations (0)

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Background

Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).

References

Smits, K.M., et al. Ann Epidemiol 20(5):401-404(2010)
Tian, X., et al. Mol. Cell. Biol. 30(5):1269-1284(2010)
Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008)
Zhao, Y., et al. Mol. Endocrinol. 20(9):2187-2198(2006)
McLellan, A.S., et al. Mech. Dev. 119 SUPPL 1, S285-S291 (2002) :

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Cat# AP10649B
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(40 western blots)
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