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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   ARV1 Antibody (N-term)   

ARV1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - ARV1 Antibody  (N-term) AP10655a
    Overlay histogram showing A2058 cells stained with AP10655A(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10655A, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - ARV1 Antibody  (N-term) AP10655a
    Overlay histogram showing A2058 cells stained with AP10655A(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10655A, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - ARV1 Antibody  (N-term) AP10655a
    All lanes : Anti-ARV1 Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: human kidney lysate Lane 3: HL-60 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ARV1 Antibody  (N-term) AP10655a
    Anti-ARV1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ARV1 Antibody  (N-term) AP10655a
    ARV1 Antibody (N-term) (Cat. #AP10655a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARV1 antibody detected the ARV1 protein (arrow).
  • WB - ARV1 Antibody  (N-term) AP10655a
    ARV1 Antibody (N-term) (Cat. #AP10655a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ARV1 antibody detected the ARV1 protein (arrow).
  • IHC-P - ARV1 Antibody  (N-term) AP10655a
    ARV1 antibody (N-term) (Cat. #AP10655a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARV1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q9H2C2
Other Accession Q3SZW3, NP_073623.1
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31052 Da
Antigen Region 23-51 aa
Additional Information
Gene ID 64801
Other Names Protein ARV1, hARV1, ARV1
Target/Specificity This ARV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human ARV1.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARV1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARV1
Function Plays a role as a mediator in the endoplasmic reticulum (ER) cholesterol and bile acid homeostasis (PubMed:11063737, PubMed:12145310, PubMed:20663892). Participates in sterol transport out of the ER and distribution into plasma membranes (PubMed:20663892).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Ubiquitous. Highly expressed in liver and adipose.
Research Areas
Citations (0)

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Background

May act as a mediator of sterol homeostasis (Potential).

References

Tong, F., et al. J. Biol. Chem. 285(44):33632-33641(2010)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Swain, E., et al. J. Biol. Chem. 277(39):36152-36160(2002)
Tinkelenberg, A.H., et al. J. Biol. Chem. 275(52):40667-40670(2000)

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$ 295.00
$ 99.00
Cat# AP10655a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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