LYRM4 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q9HD34 |
Other Accession | NP_065141.3 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 10758 Da |
Antigen Region | 43-69 aa |
Gene ID | 57128 |
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Other Names | LYR motif-containing protein 4, LYRM4, C6orf149, ISD11 |
Target/Specificity | This LYRM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-69 amino acids from the Central region of human LYRM4. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | LYRM4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | LYRM4 (HGNC:21365) |
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Synonyms | C6orf149, ISD11 |
Function | Stabilizing factor, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with NDUFAB1, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed:31664822, PubMed:17331979). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). May also participates in the iron-sulfur protein biogenesis in the cytoplasm through its interaction with the cytoplasmic form of NFS1 (PubMed:19454487). |
Cellular Location | Mitochondrion. Nucleus |
Tissue Location | Reduced mRNA levels in Friedreich ataxia patients. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Required for nuclear and mitochondrial iron-sulfur protein biosynthesis.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Henckaerts, L., et al. Clin. Gastroenterol. Hepatol. 7(9):972-980(2009)
Shi, Y., et al. Hum. Mol. Genet. 18(16):3014-3025(2009)
Weersma, R.K., et al. Am. J. Gastroenterol. 104(3):630-638(2009)
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