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NFKBIL1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - NFKBIL1 Antibody (Center) AP10682c
    Confocal immunofluorescent analysis of NFKBIL1 Antibody (Center)(Cat#AP10682c) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - NFKBIL1 Antibody (Center) AP10682c
    NFKBIL1 Antibody (Center) (Cat. #AP10682c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NFKBIL1 antibody detected the NFKBIL1 protein (arrow).
    detail
  • FC - NFKBIL1 Antibody (Center) AP10682c
    NFKBIL1 Antibody (Center) (Cat. #AP10682c) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, WB, E
Primary Accession Q9UBC1
Other Accession NP_001138434.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 43257 Da
Antigen Region 256-285 aa
Additional Information
Gene ID 4795
Other Names NF-kappa-B inhibitor-like protein 1, Inhibitor of kappa B-like protein, I-kappa-B-like protein, IkappaBL, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1, NFKBIL1, IKBL
Target/Specificity This NFKBIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human NFKBIL1.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFKBIL1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NFKBIL1
Synonyms IKBL
Function Involved in the regulation of innate immune response. Acts as negative regulator of Toll-like receptor and interferon-regulatory factor (IRF) signaling pathways. Contributes to the negative regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous proinflammatory stimuli.
Cellular Location Nucleus. Note=Nuclear localization with a speckled expression pattern in some cells. Colocalizes with CACTIN in the nucleus
Tissue Location Detected in different cell types including monocytes, T-cells, B-cells and hepatocytes
Research Areas
Citations (0)
citation

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Background

This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

References

Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Owecki, M.K., et al. Pol. Merkur. Lekarski 28(167):366-370(2010)

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$ 182.50
$ 70.00
Cat# AP10682c
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