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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   IL12_2 Antibody (C-term)   

IL12_2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - IL12_2 Antibody (C-term) AP10687B
    Overlay histogram showing A431 cells stained with AP10687b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10687b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - IL12_2 Antibody (C-term) AP10687B
    All lanes : Anti-IL12RB2 Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IL12_2 Antibody (C-term) AP10687B
    IL12RB2 Antibody (C-term) (Cat. #AP10687b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IL12RB2 antibody detected the IL12Cat. #2 protein (arrow).
  • IHC-P - IL12_2 Antibody (C-term) AP10687B
    IL12RB2 Antibody (C-term) (Cat. #AP10687b) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsils tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12RB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99665
Other Accession NP_001550.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 97135 Da
Antigen Region 756-783 aa
Additional Information
Gene ID 3595
Other Names Interleukin-12 receptor subunit beta-2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12RB2, IL12RB2
Target/Specificity This IL12_2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 756-783 amino acids from the C-terminal region of human IL12_2.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL12_2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL12RB2
Function Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Isoform 2 is expressed at similar levels in both naive and activated T-cells.
Research Areas
Citations (0)

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The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.


Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Liu, X., et al. Nat. Genet. 42(8):658-660(2010)
Mizuki, N., et al. Nat. Genet. 42(8):703-706(2010)
Remmers, E.F., et al. Nat. Genet. 42(8):698-702(2010)

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$ 295.00
$ 99.00
Cat# AP10687B
(40 western blots)
Availability: In Stock
Bulk Size
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