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UBE2M Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1068a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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UBE2M Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | P61081 |
| Other Accession | Q6DCZ9, P61082, A3KN22, NP_003960 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Xenopus |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 20900 Da |
UBE2M Antibody (N-term) - Additional info | |
| Gene ID 9040 | |
| Other Names UBE2M; UBC12; NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M | |
| Target/Specificity This UBE2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~37 amino acids from the N-terminal region of human UBE2M. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions UBE2M Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
UBE2M Antibody (N-term) - Protein Information | |
| Name UBE2M | |
| Synonyms UBC12 | |
| Function Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation | |
UBE2M Antibody (N-term) - Related products
AP1068a: UBE2M Antibody (N-term)
AP1068b: UBE2M Antibody (C-term)
AP2169a: UBE2M Antibody (N-term K11)
AP2169b: UBE2M Antibody (Center)
RI15581: UBE2M predesign siRNA
DC08146: Human UBE2M cDNA Clone
LY11934a: UBE2M Over-expression Lysate
BP1068a: UBE2M Antibody (N-term) Blocking Peptide
BP1068b: UBE2M Antibody (C-term) Blocking Peptide
BP2169a: UBE2M Antibody (N-term K11) Blocking Peptide
UBE2M Antibody (N-term) - Application data
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Western blot analysis of anti-UBE2M Antibody (N-term)(Cat.#AP1068a) in A2058 cell line lysates (35ug/lane). UBE2M(arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of UBE2M (arrow) using rabbit polyclonal UBE2M Antibody (E15) (Cat. #AP1068a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2M gene.
UBE2M Antibody (N-term) - Research Areas
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BACKGROUND
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
REFERENCES
Gong, L., et al., J. Biol. Chem. 274(17):12036-12042 (1999). Osaka, F., et al., Genes Dev. 12(15):2263-2268 (1998).
