|Application ||WB, FC, E|
|Calculated MW||147810 Da|
|Antigen Region||630-656 aa|
|Other Names||Regulator of nonsense transcripts 2, Nonsense mRNA reducing factor 2, Up-frameshift suppressor 2 homolog, hUpf2, UPF2, KIAA1408, RENT2|
|Target/Specificity||This UPF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 630-656 amino acids from the Central region of human UPF2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UPF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.|
|Cellular Location||Cytoplasm, perinuclear region|
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Provided below are standard protocols that you may find useful for product applications.
UPF2 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p.
Clerici, M., et al. EMBO J. 28(15):2293-2306(2009)
Cronin, S., et al. Eur. J. Hum. Genet. 17(2):213-218(2009)
Woeller, C.F., et al. EMBO Rep. 9(5):446-451(2008)
Chamieh, H., et al. Nat. Struct. Mol. Biol. 15(1):85-93(2008)
Singh, G., et al. Mol. Cell 27(5):780-792(2007)
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