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AMY2B Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP10694a 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP10694a-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
  • Specification
  • Citiations : 0
  • Reviews
  • Protocols
  • Backgrounds

AMY2B Antibody (N-term) - Product info

ApplicationWB, IHC, FC, IF
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP19961
Other AccessionP04746, P04745, NP_066188.1
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW57710 Da

AMY2B Antibody (N-term) - Additional info

Gene ID 280
Other Names
AMY2B; Alpha-amylase 2B; 1, 4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase
Target/Specificity
This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human AMY2B.
Dilution
WB~~1:100~500
IHC~~1:50~100
FC~~1:10~50
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
AMY2B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

AMY2B Antibody (N-term) - Protein Information

Name AMY2B
Cellular Location
Secreted (By similarity).

AMY2B Antibody (N-term) - Related products

AP10694a: AMY2B Antibody (N-term)

DC03076: Human AMY2B cDNA Clone

LY12224a: AMY2B Over-expression Lysate

AMY2B Antibody (N-term) - Application data

  • AMY2B Antibody (N-term) (Cat. #AP10694a) western blot analysis in HL-60,CEM and K562 cell line lysates (35ug/lane).This demonstrates the AMY2B antibody detected the AMY2B protein (arrow).

  • AMY2B antibody (N-term) (Cat. #AP10694a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

  • AMY2B Antibody (N-term) (Cat. #AP10694a) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Confocal immunofluorescent analysis of AMY2B Antibody (N-term) (Cat#AP10694a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

AMY2B Antibody (N-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

REFERENCES

Baltova, S.D. Folia Med (Plovdiv) 47(1):37-41(2005) Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004) : Koyama, I., et al. Clin. Chim. Acta 309(1):73-83(2001) Aughsteen, A.A. Saudi Med J 22(5):410-415(2001) Omichi, K., et al. Biochim. Biophys. Acta 1203(2):224-229(1993)