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AMY2B Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - AMY2B Antibody (N-term) AP10694a
    Confocal immunofluorescent analysis of AMY2B Antibody (N-term) (Cat#AP10694a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - AMY2B Antibody (N-term) AP10694a
    AMY2B Antibody (N-term) (Cat. #AP10694a) western blot analysis in HL-60,CEM and K562 cell line lysates (35ug/lane).This demonstrates the AMY2B antibody detected the AMY2B protein (arrow).
    detail
  • IHC-P - AMY2B Antibody (N-term) AP10694a
    AMY2B antibody (N-term) (Cat. #AP10694a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMY2B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - AMY2B Antibody (N-term) AP10694a
    AMY2B Antibody (N-term) (Cat. #AP10694a) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, IHC-P, WB, E
Primary Accession P19961
Other Accession P04746, P04745, NP_066188.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57710 Da
Antigen Region 74-100 aa
Additional Information
Gene ID 280
Other Names Alpha-amylase 2B, 4-alpha-D-glucan glucanohydrolase 2B, Carcinoid alpha-amylase, AMY2B
Target/Specificity This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMY2B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMY2B
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

References

Baltova, S.D. Folia Med (Plovdiv) 47(1):37-41(2005)
Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004) :
Koyama, I., et al. Clin. Chim. Acta 309(1):73-83(2001)
Aughsteen, A.A. Saudi Med J 22(5):410-415(2001)
Omichi, K., et al. Biochim. Biophys. Acta 1203(2):224-229(1993)

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$ 365.00
$ 140.00
Cat# AP10694a
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