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>   home   >   Products   >   Primary Antibodies   >   ARHGAP10 Antibody (Center)   

ARHGAP10 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ARHGAP10 Antibody (Center) AP10695b
    ARHGAP10 Antibody (Center) (Cat. #AP10695b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ARHGAP10 antibody detected the ARHGAP10 protein (arrow).
  • IHC-P - ARHGAP10 Antibody (Center) AP10695b
    ARHGAP10 antibody (Center) (Cat. #AP10695b) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP10 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ARHGAP10 Antibody (Center) AP10695b
    ARHGAP10 Antibody (Center) (Cat. #AP10695b) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession A1A4S6
Other Accession Q08DP6, NP_078881.3
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 89375 Da
Antigen Region 237-263 aa
Additional Information
Gene ID 79658
Other Names Rho GTPase-activating protein 10, GTPase regulator associated with focal adhesion kinase 2, Graf-related protein 2, Rho-type GTPase-activating protein 10, ARHGAP10, GRAF2
Target/Specificity This ARHGAP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-263 amino acids from the Central region of human ARHGAP10.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARHGAP10 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARHGAP10
Synonyms GRAF2
Function GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).
Cellular Location Cytoplasm. Cytoplasm, perinuclear region. Cell membrane Note=Association to cell membrane is dependent on PH domain
Tissue Location High levels of expression in heart and skeletal muscle.
Citations (0)

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Background

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Azzato, E.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(4):1140-1143(2010)
Lind, P.A., et al. Twin Res Hum Genet 13(1):10-29(2010)
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010)
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)

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Cat# AP10695b
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(40 western blots)
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