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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP21 Antibody (N-term)   

USP21 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - USP21 Antibody (N-term) AP1069a
    Western blot analysis of anti-USP21 Antibody (N-term) (Cat. AP1069a) in Hela cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.
  • IHC-P - USP21 Antibody (N-term) AP1069a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9UK80
Other Accession B2GUX4, Q9QZL6, Q2KJ72
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62656 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 27005
Other Names Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23
Target/Specificity This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human USP21.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP21 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP21
Synonyms USP23
Function Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.
Cellular Location Cytoplasm. Nucleus
Tissue Location Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung
Research Areas
Citations ( 0 )

Background

USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.

References

Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000).
Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).
Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).

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$ 295.00
$ 99.00
Cat# AP1069a
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Quantity:
(40 western blots)
Availability: In Stock
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