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USP21 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1069a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
USP21 Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q9UK80 |
| Other Accession | B2GUX4, Q9QZL6, Q2KJ72 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 62656 Da |
USP21 Antibody (N-term) - Additional info | |
| Gene ID 27005 | |
| Other Names USP21; USP23; Ubiquitin carboxyl-terminal hydrolase 21; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21 | |
| Target/Specificity This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human USP21. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions USP21 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
USP21 Antibody (N-term) - Protein Information | |
| Name USP21 | |
| Synonyms USP23 | |
| Function Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity) Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates | |
| Cellular Location Cytoplasm. Nucleus. | |
| Tissue Location Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung | |
USP21 Antibody (N-term) - Related products
AP1069a: USP21 Antibody (N-term)
AP2041a: USP21 Antibody (Center)
AP2147a: USP21 Antibody (C-term)
RI15650: USP21 predesign siRNA
BP1069a: USP21 Antibody (N-term) Blocking Peptide
BP2041a: USP21 Antibody (Center) Blocking Peptide
USP21 Antibody (N-term) - Application data
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Western blot analysis of anti-USP21 Antibody (N-term) (Cat. AP1069a) in Hela cell line lysates (35ug/lane). USP21 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
USP21 Antibody (N-term) - Research Areas
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BACKGROUND
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.
REFERENCES
Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000). Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996). Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).
