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CHST2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CHST2 Antibody (Center) AP10704c
    CHST2 Antibody (Center) (Cat. #AP10704c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CHST2 antibody detected the CHST2 protein (arrow).
  • FC - CHST2 Antibody (Center) AP10704c
    CHST2 Antibody (Center) (Cat. #AP10704c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4C5
Other Accession Q80WV3, NP_004258.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57857 Da
Antigen Region 309-335 aa
Additional Information
Gene ID 9435
Other Names Carbohydrate sulfotransferase 2, 282-, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2, GST-2, N-acetylglucosamine 6-O-sulfotransferase 1, GlcNAc6ST-1, Gn6ST-1, CHST2, GN6ST
Target/Specificity This CHST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-335 amino acids from the Central region of human CHST2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHST2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST2
Synonyms GN6ST
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.
Cellular Location Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein
Tissue Location Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes.
Citations (0)

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N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).


Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Desko, M.M., et al. Glycobiology 19(10):1068-1077(2009)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)
Kanoh, A., et al. Glycoconj. J. 23 (5-6), 453-460 (2006) :

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$ 295.00
$ 99.00
Cat# AP10704c
(40 western blots)
Availability: In Stock
Bulk Size
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