COX10 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q12887 |
Other Accession | NP_001294.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 48910 Da |
Antigen Region | 383-410 aa |
Gene ID | 1352 |
---|---|
Other Names | Protoheme IX farnesyltransferase, mitochondrial, 251-, Heme O synthase, COX10 |
Target/Specificity | This COX10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-410 amino acids from the C-terminal region of human COX10. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | COX10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | COX10 |
---|---|
Function | Converts protoheme IX and farnesyl diphosphate to heme O. |
Cellular Location | Mitochondrion membrane; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes heme A:farnesyltransferase, which is not a structural subunit but required for the expression of functional COX and functions in the maturation of the heme A prosthetic group of COX. This protein is predicted to contain 7-9 transmembrane domains localized in the mitochondrial inner membrane. A gene mutation, which results in the substitution of a lysine for an asparagine (N204K), is identified to be responsible for cytochrome c oxidase deficiency. In addition, this gene is disrupted in patients with CMT1A (Charcot-Marie-Tooth type 1A) duplication and with HNPP (hereditary neuropathy with liability to pressure palsies) deletion.
References
Chen, Z., et al. Oncogene 29(30):4362-4368(2010)
Vitali, M., et al. J Neural Transm 116(12):1635-1641(2009)
Dassa, E.P., et al. EMBO Mol Med 1(1):30-36(2009)
Veluthakal, R., et al. Diabetes 56(1):204-210(2007)
Coenen, M.J., et al. Ann. Neurol. 56(4):560-564(2004)
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