FBXW8 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
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Primary Accession | Q8N3Y1 |
Other Accession | NP_036306.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 67394 Da |
Antigen Region | 270-299 aa |
Gene ID | 26259 |
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Other Names | F-box/WD repeat-containing protein 8, F-box and WD-40 domain-containing protein 8, F-box only protein 29, FBXW8, FBW6, FBW8, FBX29, FBXO29, FBXW6 |
Target/Specificity | This FBXW8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the Central region of human FBXW8. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | FBXW8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | FBXW8 |
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Synonyms | FBW6, FBW8, FBX29, FBXO29, FBXW6 |
Function | Substrate-recognition component of a Cul7-RING ubiquitin- protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7- RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988). Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation (PubMed:24362026). Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (PubMed:24793695). |
Cellular Location | Cytoplasm, perinuclear region. Golgi apparatus. Note=Colocalizes with CUL7 at the Golgi apparatus in neurons. {ECO:0000250|UniProtKB:P0DL28} |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
References
Tsutsumi, T., et al. Mol. Cell. Biol. 28(2):743-751(2008)
Koch, H.B., et al. Cell Cycle 6(2):205-217(2007)
Okabe, H., et al. PLoS ONE 1, E128 (2006) :
Watanabe, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4419-4424(2004)
Dias, D.C., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16601-16606(2002)
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