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GPAA1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | O43292 |
| Other Accession | NP_003792.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 67623 Da |
| Antigen Region | 46-73 aa |
| Gene ID | 8733 |
|---|---|
| Other Names | Glycosylphosphatidylinositol anchor attachment 1 protein, GPI anchor attachment protein 1, GAA1 protein homolog, hGAA1, GPAA1, GAA1 |
| Target/Specificity | This GPAA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-73 amino acids from the N-terminal region of human GPAA1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GPAA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GPAA1 |
|---|---|
| Synonyms | GAA1 |
| Function | Component of the GPI transamidase complex, necessary for transfer of GPI to proteins (PubMed:34576938). Essential for GPI- anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
| Tissue Location | Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues |
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Provided below are standard protocols that you may find useful for product applications.
Background
Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.
References
Jiang, W.W., et al. Mol. Cancer 6, 74 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006)
Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005)
Vainauskas, S., et al. J. Biol. Chem. 279(8):6540-6545(2004)
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