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GPAA1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GPAA1 Antibody (N-term) AP10722a
    GPAA1 Antibody (N-term) (Cat. #AP10722a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the GPAA1 antibody detected the GPAA1 protein (arrow).
  • IHC-P - GPAA1 Antibody (N-term) AP10722a
    GPAA1 antibody (N-term) (Cat. #AP10722a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPAA1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - GPAA1 Antibody (N-term) AP10722a
    GPAA1 Antibody (N-term) (Cat. #AP10722a) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43292
Other Accession NP_003792.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67623 Da
Antigen Region 46-73 aa
Additional Information
Gene ID 8733
Other Names Glycosylphosphatidylinositol anchor attachment 1 protein, GPI anchor attachment protein 1, GAA1 protein homolog, hGAA1, GPAA1, GAA1
Target/Specificity This GPAA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-73 amino acids from the N-terminal region of human GPAA1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPAA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPAA1
Synonyms GAA1
Function Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
Citations (0)

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Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.


Jiang, W.W., et al. Mol. Cancer 6, 74 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006)
Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005)
Vainauskas, S., et al. J. Biol. Chem. 279(8):6540-6545(2004)

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$ 295.00
$ 99.00
Cat# AP10722a
(40 western blots)
Availability: In Stock
Bulk Size
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