|Application ||WB, IHC-P, FC, E|
|Calculated MW||150528 Da|
|Antigen Region||586-613 aa|
|Other Names||Inhibitor of Bruton tyrosine kinase, IBtk, IBTK, BTKI, KIAA1417|
|Target/Specificity||This IBTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-613 amino acids from the Central region of human IBTK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||IBTK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription.|
|Cellular Location||Cytoplasm. Membrane; Peripheral membrane protein. Note=Translocates to the plasma membrane upon IgM stimulation|
|Tissue Location||Expressed in DeFew, HEK293T, HeLa and in Jurkat, MC3 and NB4 lymphoid cells (at protein level). Isoform 1 is the predominant isoform expressed in all examined tissues and cell lines. Highly expressed in hemopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes and bone marrow). Weakly or not expressed in other tissues|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription.
Fiume, G., et al. Comput Biol Chem 33(6):434-439(2009)
Spatuzza, C., et al. Nucleic Acids Res. 36(13):4402-4416(2008)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
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